The United States considers Botswana an advocate of and a model for stability in Africa and has been a major partner in Botswana's development since its independence. The U.S. Peace Corps closed out its presence in Botswana on December 1997, bringing to an end 30 years of well-regarded assistance in education, business, health, agriculture, and the environment. Similarly, the USAID phased out a longstanding partnership with Botswana in 1996, after successful programs emphasizing education, training, entrepreneurship, environmental management, and reproductive health. Botswana, however, continues to benefit along with its neighbors in the region from USAID's Initiative for Southern Africa and the USAID Regional Center for Southern Africa is headquartered in Gaborone. The United States International Board of Broadcasters (IBB) operates a major Voice of America (VOA) relay station in Botswana serving most of the African Continent.

In 1995, the Centers for Disease Control (CDC) started the BOTUSA Project in collaboration with the Botswana Ministry of Health in order to generate information to improve TB control efforts in Botswana and elsewhere in the face of the TB and HIV/AIDS co-epidemics. Under the 1999 U.S. Government's Leadership and Investment in Fighting an Epidemic (LIFE) Initiative, CDC through the BOTUSA Project has undertaken many projects and has assisted many organizations in the fight against the HIV/AIDS epidemic in Botswana.

The Governments of Botswana and the United States entered into an agreement in July 2000 to establish an International Law Enforcement Academy (ILEA) in Gaborone. The academy, jointly financed, managed and staffed by the two nations, provides training to police and government officials from Southern Africa and eventually from across the continent. The academy's permanent campus, in Otse outside of Gaborone, is scheduled to open in late 2002.

The U.S. Embassy is on Embassy Drive off Khama Crescent--P. O. Box 90, Gaborone (tel. 267-353-982; fax 267-356-947). USAID is located on Lebatlane Road. DAO and ODC are located at the embassy. CDC is located on Ditlhakore Way in Gaborone. ILEA will be located in Otse, about 30 minutes outside of Gaborone. The IBB station is located in Selebi-Phikwe, about 400 kilometers northeast of Gaborone.