A number of 2-year and community colleges offer associate degree programs that prepare graduates to work as teacher assistants. However, most teacher assistants receive on-the-job training. Those who tutor and review lessons with students must have a thorough understanding of class materials and instructional methods, and should be familiar with the organization and operation of a school. Teacher assistants also must know how to operate audiovisual equipment, keep records, and prepare instructional materials, as well as have adequate computer skills.

Teacher assistants should enjoy working with children from a wide range of cultural backgrounds, and be able to handle classroom situations with fairness and patience. Teacher assistants also must demonstrate initiative and a willingness to follow a teacher's directions. They must have good writing skills and be able to communicate effectively with students and teachers. Teacher assistants who speak a second language, especially Spanish, are in great demand for communicating with growing numbers of students and parents whose primary language is not English.

Advancement for teacher assistants—usually in the form of higher earnings or increased responsibility—comes primarily with experience or additional education. Some school districts provide time away from the job or tuition reimbursement so that teacher assistants can earn their bachelor's degrees and pursue licensed teaching positions. In return for tuition reimbursement, assistants are often required to teach a certain length of time for the school district.